短小棒状杆菌体外激活大鼠单核巨噬细胞抗肿瘤作用的研究

IN VITRO ANTI-TUMOR ACTIVITY OF RAT MONOCYTES AND MACROPHAGES ACTIVATED BY CORYNEBACTERIUM PARVUM

从同只大鼠体内分离出高纯度的单核细胞、肝枯否细胞以及脾、肺和腹腔巨噬细胞,以小鼠淋巴瘤细胞与人肝癌细胞为靶细胞。应用[~3H]TdR 参入实验,比较了5种效应细胞对2种靶细胞自然增殖的抑制效应,以及短小棒状杆菌(CP)对效应细胞抗瘤作用的影响。结果如下:1.5种细胞均具有明显的自然抑制肿瘤细胞增殖的效应;2.单核细胞对2种靶细胞增殖的抑制程度无明显差别,其他4种巨噬细胞分别对2种靶细胞的作用,则有显著性差异;5种细胞相比,单核细胞对淋巴瘤的抑制作用最强,枯否细胞对肝癌细胞的抑制作用最强;3.CP 有增强5种效应细胞的抗淋巴瘤作用,分别提高140～408%不等;4.CP 对5种效应细胞的抗肝癌作用,则表现为抑制效果,分别下降48～64%。本研究结果表明,单核巨噬细胞系统具有选择性的抗肿瘤潜能,故在应用免疫制剂增强巨噬细胞的抗肿瘤作用中,需针对不同的肿瘤选用适宜的制剂。

Monocytes,Kupffer cells,spleen macrophages,lung and peritoneal macrophages were isolated from same rat simultaneously.The effector cells from diverse anato- mical sites were checked for their tumor cytostatic properties using an in vitro tar- get cell growth inhibition assay through measuring (~3H)-TdR incorporation into tumor cell DNA.All of them expressed spontaneous cytostatic activity against hu- man SMMC-7721 hepatoma and mouse YAC-1 lymphoma cells.The effector cells, except monocytes,were shown to exert inhibition on various target cells with diffe- rences in their susceptibility.The cytostasis against either tumor cell lines varied with five types of effector cells.The most effective cells for inhibiting the growth of lymphoma were monocytes,and Kupffer cells for heptoma,The effector cells stimulated by Corynebacterium parvum (CP) in vitro were shown that their cytostatic activity against YAC-1 was increased up to 140-408%,but to heptoma,it was decreased to 48-64%.Furthermore,the efficiency of CP stimulation varied with different effector cells also.It is concluded that monocytes and macrophages with such natural selective tumor cytostatic capacity were thus everywhere readily availa- ble for activation.CP may enhance or inhibit the cytostatic effect of monocytes and macrophages on diverse tumor cell lines.It might suggest that the use of CP in the tumor immunotherapy should be considered with different type of tumors.