畜禽肌肉、内脏及虾中磺胺二甲嘧啶残留量的检测技术研究

Study on the determination of sulfamethazine residue in muscle and viscera of livestock,poultry and shrimp

为了快速、简便、灵敏地监测畜禽肌肉、内脏及虾中的磺胺二甲嘧啶残留 ,建立了高效液相色谱法快速检测技术。动物组织中的磺胺二甲嘧啶用二氯甲烷超声提取 3次 ,提取液与 2 %硫酸钠水溶液进行液 -液分配 ,下层有机相经脱水、浓缩后用 0 0 2mol L盐酸溶解浓缩瓶中残留物 ,以正己烷去除脂溶性杂质 ,经 0 4 5 μm滤膜过滤后供HPLC测定。结果表明本方法的线性范围为 0 2～ 3 0 μg mL ,方法的最低检出浓度为 2 1μg kg ,在 5 0～ 10 0 μg kg的加标水平 ,方法平均回收率为76 6 %～ 93 6 % ,相对标准偏差为 4 4 %～ 8 4 %。该方法适用范围广 ,回收率高 ,检测限低 ,简单易行 ,易于在基层推广应用。

A rapid,simple and sensitive high performance liquid chromatographic method for the determination of sulfamethazive residue in muscle and viscera of the livestock,poultry and shrimp has been established.The sulfamethazine in animal tissues was extracted three times by dichloromethane with ultrasonic.The extract was cleaned up with 2% Na\-2SO\-4 solution,dehydrated with Na\-2SO\-4,and then concentrated.The condensate was dissolved by 0.02 mol/L HCl solution and further cleaned up by n-hexane.The purified solution was examined at UV 263 nm by HPLC.The detection limit of this method was 2.1 μg/kg,the recovery was 76.6%～93.6%, RSD was 4.4%～8.4%.This method can be widely applied because of its high recovery,low detection limit and simple procedure.