CCD-二极管阵列分光光度法同时测定食品中的维生素B_1和维生素B_2

Simultaneous spectrophotometric determination of vitamin B_1 and vitamin B_2 in food by charge-coupled device-diode array detection

为建立一种同时测定食品中维生素B1和维生素B2 的简单快速的方法 ,在氢氧化钠和碳酸氢钠混合溶液缓冲介质中 ,对氨基苯磺酸重氮盐与维生素B1发生偶氮反应 ,维生素B2 不发生反应 ,采用电感耦合二极管阵列检测装置进行检测 ,用偏最小二乘法解析重叠光谱 ,从而实现了对食品中维生素B1、维生素B2 的同时测定。方法的线性范围维生素B1和维生素B2 分别为 0～ 0 0 5 0mg mL和 0～ 0 10mg mL ;检出限分别为 0 0 0 72mg mL和 0 0 0 85mg mL。平均加标回收率维生素B1为 87 3%～ 10 5 4 % ,维生素B2 为 90 0 %～ 10 4 9% ;相对标准偏差维生素B1为 3 6 %～ 9 1% ,维生素B2 为 4 7～ 8 0 %。该方法简单、快速、测定干扰小 ,结果与国家标准测定方法的差异无显著性。

Objectives:To search for a simple,rapid method for simulataneous determination of vitamin B \-1 and vitamin B \-2 in foods.Methods:CCD diode array detection-spectrophotometric setup was established for simultaneous determination of vitamin B\-1 and vitamin B \-2 with azo-reaction between diazosalt of sulfanilic acid and vitamin B \-1 in solution of NaOH and NaHCO_3. Results:The linear range of vitamin B \-1 and vitamin B \-2 was 0～0.050 mg/mL and 0～0.10 mg/mL,respectively.The detection limit was 0.007 2 mg/mL for vitamin B \-1 and 0.008 5 mg/mL for vitamin B \-2.The recovery of spiked samples was from 87.3% to 105.4%,and the relative standard deviation was less than 9.1%.Conclusions:The method was proved to be more simple,more rapid and less interfered by metal ions.The results obtained checked very well with that by standard method.