国产环硫雄醇对大鼠子宫组织及蜕膜瘤影响的组织化学研究

HISTOCHEMICAL STUDY OF THE EFFECTS OF THE EPITIOSTANOL ON THE UTERUS AND DECIDUOMA OF RATS

环硫雄醇为抗乳癌药,同时具有明显的抗着床作用。为了探讨该药抗生殖作用机理,本研究采用成年,有生育力的雌性大白鼠22只,摘除卵巢后,对照组先后给予雌二醇和18—甲基炔诺酮,实验组除按对照组给药外,在给18—甲基炔诺酮的同时,给予环硫雄醇。每只动物右侧子宫角穿线诱发蜕膜瘤。先后用药共13天后解剖观察子宫并称重。子宫组织分别作碱性磷酸酶、酸性磷酸酶、β-葡萄糖苷酸酶、多糖类和DNA、RNA等组织化学研究实验组动物子宫明显增粗,蜕膜瘤大,组织观察可见子宫壁厚,内膜上皮、腺体、基质细胞和平滑肌分化较好,组织化学显示碱性磷酸酶、酸性磷酸酶和β-葡萄苷酸酶活性明显减弱。蜕膜瘤细胞的糖元、基质细胞RNA较丰富。对照组三种酶的活性较强,但组织分化较低。本文提出环硫雄醇具有抑制雌激素激发碱性磷酸酶等活性作用,并可能具有促进孕激素与受体结合的效应。其抗着床作用可能与子宫酶活性受到抑制,从而干扰早胚和子宫组织的代谢过程有关。

Epitiostanol is a anticarcinome drug and have obvious offects of antireproduction function in the femal animal. The aim of this study is to detect the mechanism of antireproduction. 22 Wistar strain female rats were ovariectomized. then, estradial and 18-methyl-nore-thiodronum wereinjected in muscle of the rats by turn in the control group. In experimental animal group, in addition to same treatment as the control group also injedted with Epitiostanol. The deciduoma of the uterus were induced in all animals. After chemical ireatment, acid phosphotase, alkaline phosphatase, β-GU (glucuronidase) and mucoprotein in the uterus of rats were researched in each histochemical mehtod. The diameter of the uterus were obvious increase, the deciduoma were larger in the experimental group. The epithelium of endometerium, uterine glands, the stromal cells and smooth mluscle, their development and differenation were more well. The value of glucogen in deciduoma were more than the control group. The activity of AGP, ALP, and β-GU in the experimental group had obvious decrease. The authors of this study presumed Epitiostanol was possible to prompt on combine between progesterone and receptor in the ulerus. Inhibited enzymes activity in uterus by Epitiostanol mey be one of the mechanism of antiimplanation and antipregnancy in early. These results were perhaps related through distubance on the metabolism in early embryo and the uterine tissue.