锯缘青蟹不同器官组织中总抗氧化能力和SOD活性的比较研究

Comparative study on total antioxidative capabilityand SOD activity in different organs and tissues of mud crab Scylla serrata

采用生化测定的方法,对锯缘青蟹的鳃、肝胰腺和肌肉等不同器官组织中的总抗氧化能力和超氧化物歧化酶活性进行了测定.结果表明,不同器官组织中的总抗氧化能力和超氧化物歧化酶活性各异.总抗氧化能力由强至弱依次为肝胰腺>肌肉>鳃,分别为71.16±17.75U/mg蛋白,28.55±4.45U/mg蛋白,19.82±3.20U/mg蛋白.而超氧化物歧化酶活性由强至弱则依次为肝胰腺>鳃>肌肉,分别为38.62±5.24 NU/mg蛋白,27.08±4.79 NU/mg蛋白,6.33±1.54 NU/mg蛋白.统计分析显示,各器官组织间的总抗氧化能力和超氧化物歧化酶活性有显著性差异(p<0.01),这与不同器官组织的生理功能是密切相关的.

This study was to determine total antioxidative capability and Superoxide dismutase (SOD) activity in different organs and tissues of mud crab, Scylla serrata by the biochemiscal method. The results showed the difference of total antioxidative capability or SOD activity among different organs and tissues. Among them,total antioxidative capability is hepatopancreas> muscle > gill in descending order, the value is 71.16 ± 17. 75U/mg prot,28. 55 ± 4.45U/mg prot and 19.82 ± 3. 20U/mg prot, respectively. Superoxide dismutase activity is hepatopancreas > gill > muscle in descending order, the value is 38. 62 ± 5. 24 NU/mg prot,27. 08 ±4.79 NU/mg prot and 6.33 ± 1.54 NU/mg prot respectively. Statistics assay indicates that there is significant difference among the total antioxidative capability or Superoxide dismutase (SOD) activity of different organs and tissues (p<0.01) .This is due to the different physiological function conducted by organs and tissues.