肝缺血再灌注损伤和氯丙嗪的保护作用—黄嘌呤脱氢酶活性的组化观察

HEPATIC ISCHEMIA-REPERFUSION INJURY AND THE PROTECTIVE EFFECT BY CHLORPROMAZINE——THE HISTOCHEMICAL OBSERVATION OF XANTHINE DEHYDROGENASE ACTIVITY

本文用组织化学方法观察大鼠肝脏缺血不同时间后再灌注时对黄嘌呤脱氢酶(Xanthlne Dehydrogenase简称XDase)活性的影响,同时观察氯丙嗪的保护作用。结果表明,短时间(0.5小时)肝缺血后再灌注,XDase活性和HE染色都无异常改变。长时间(2小时)肝缺血,达到不可逆性损伤后再灌注3小时,可使XDase活性明显减低,再灌注24小时后,HE染色有大面积肝细胞凝固性坏死,氯丙嗪对这种改变有明显的保护作用。

By histochemical means, we observed the effects of reperfusion on xanthine dehydrogenase (XDase) activity after hepatic ischemia in different times; moreover, we observed the protective effect by chlorpromazine. Results showed that the reperfusion after short periods of hepatic ischemia(0.5 hours),XDase activity and HE colouring were close to control group; if hepatic ischemia was as long as 2 borers, XDase activity was evidently lower after 3 hour reperfusion and HE colouring showed a larger areas of necrosis in rat liver after 24 hour reperfusion. Chlorpromazine has a profound protective effect against the ischemia-reperfusion injury to rat liver.