尾部悬吊对雄性大鼠生殖的影响

Effects of Simulated Weightlessness on Reproduction in Male Rats.

目的探讨尾部悬吊对雄性大鼠生殖腺及生殖激素的影响。方法雄性大鼠尾部悬吊及恢复重力后 ,测量睾丸重量、HE染色后观察其组织形态变化 ,观察附着精子有无 ,放免法测睾酮含量 ,免疫组织法检测垂体LH β亚单位阳性细胞率。 结果睾丸重量极显著减轻、曲精细管明显萎缩、精母细胞明显减少 ,附睾未发现精子 ,睾酮含量极显著降低 ,垂体细胞LH β亚单位阳性细胞数量显著增加。 结论尾部悬吊对雄性大鼠性腺及生殖功能有负面影响。

Objective To study the effects of tail suspension on gonad and spermatic hormone in male rats. Method After tail suspension and reambulation some times in male rats,weight of testis was measured,sperm of epididymis was inspected,morphology of testicular cell was compared,and antent of testosterone assay by radioimmunoassay ratio of LH β positive cell in pituitary was measured by immunohistochemic.Result After tail suspension,weight of testis in male rats decreased significantly.Shape of the convoluted tubules and number of all levels of the spermatocyte in testis atrophied markedly.No sperm was found in the epididymis.Content of testosterone decreased markedly.Number of positive cell of β subunit of LH in the pituitary increased markedly.Conclusion Tail suspension has negative effect on sex gland and reproduction function of male rats.