摘要
本文报道了白细胞介素-2(IL-2)刺激ConA(5μg/ml)活化的小鼠T细胞产生的条件培养液(TCM)中含有CFU-GEMM诱导活性。这种CFU-GEMM诱导活性的生成在IL-2作用后48h达到高峰。特异性抗IL-3单克降抗体可以完全中和该条件培养液中的CFU-GEMM诱导活性。进一步证明,TCM可以刺激IL-3依赖细胞系FDC-P_1细胞的增殖;在IL-2作用于ConA活化的T细胞后可促进其细胞表达高水平的IL-3mRNA。这些结果表明IL-2可以加强小鼠T细胞产生IL-3。
In addition to the regulation of T cell growth, IL-2 exerts effects on the induction of certain lymphokineso We show here that IL-2 synergizes with 5μg/ml of ConA to promote the production of IL-3 in mouse splenic T cell cultures. IL-3 was measured as CFU-GEMM-inducing activity on mouse bone marrow progenitor cells in the supernatant of the stimulated mouse splenic T cells (TCM). The resting T cells produced no CFU-GEMM-inducing activity, but could be induced to produce low level of CFUGEMM-indueing activity in the presence of ConA. In vitro exposure to IL-2 markedly increased CFU-GEMM-inducing activity production (nearly up to 8-fold) by the ConAactivated T cells. Optimal stimulation was observed when 80 u/ml IL-2 was used for 48 h incubation. Anti-mouse IL-3 monoclonal antibody inhibited the CFU-GEMM inducing activity of TCM. Moreover, the TCM stimulated the proliferation of IL-3 dependent cell line FDC-P_1. We also show that IL-2 and ConA-treated T cells expressed high level of IL-3 mRNA through dot blot analysis. These results confirmed the nature of CFU-GEMM-inducing activity of TCM as IL-3. The capacity of IL-2 to promote the production of IL-3 may represent an important mechanism by which it mediate the communication between the immune and hematopoietic systems.
出处
《实验生物学报》
CSCD
1992年第1期25-30,共6页
Acta Biologiae Experimentalis Sinica
基金
国癌症协会研究基金
