安祖花人工种子的研制

STUDIES ON THE MANUFACTURE OF ARTIFICIAL SEEDS OF Anthurium andraeanum

用安祖花叶片作为外植体,诱导产生致密愈伤组织,将安祖花致密愈伤组织切成规则小块,用(MS+0.1 mg/L NAA)培养基预培养半个月,诱导根的发生。再用继代培养基包埋培养物进行固定化培养,经1月后筛选具不定根的培养物,采用再包埋方法制成人工种子。当这种人工种子播于有菌土壤时可得到63%的成活率(转株率)。我们认为致密愈伤组织中不定芽和愈伤组织间发生的是生物学接触,不定芽的初始生长由愈伤组织通过生物学途径提供营养,从而大大提高了人工种子的活力。用含不定芽的致密愈伤组织作为培养物,对于人工种子的机械化生产和田间应用具有参考价值。

Tight calli were induced from leaf explants of Anthurium andraeanum, were splited into small equal masses used as culture materials. Culture materials were precultured on MS medium containing 0.1 mg/L NAA to induce adventitious roots, after half an month, they were encapsulated with maintenance medium and were immobolization cultured on 1/2MS medium for one month, the culture materials with adventitious root were sifted and re-encapsulated into artificial seeds. This kind of artificial seeds were directly sown in nonsterile soil, as a result, 63% survival rate (seedling rate) was obtained under nonsterile condition. We consider that the biological contact take place between the callus and adventitious shoot in biological way in the beginning of adventitious shoot development, so the artificial seed vigor was developed greatly. Manufacture of artificial seed with tight callus will be a useful method to mechanical production and field application.