哮喘患儿淋巴细胞糖皮质激素受体、T细胞亚群及血清免疫球蛋白的研究

The Research of Lymphocyte Glucocorticoid Receptor. T Cell Subpopulation and Serum Immunoglobulin in Child Patients with Asthma

研究目的 探讨淋巴细胞糖皮质激素受体、细胞亚群及血清免疫球蛋白与儿童哮喘的关系。 研究设计 病例对照研究 患者和其他参与者 哮喘患儿34例(观察组),对照组34例,性别组成与年龄分布与观察组相同,均为健康儿童。 处理方法 所有患者及参与者均于晨8时外周或股静脉取血,肝素抗凝待测。 测定和主事结果 用受体放射配体结合分析法测定淋巴细胞糖皮质激素受体(GCR),用微量细胞毒法检测T细胞亚群,用半自动生化分析仪测定血清中的免疫球蛋白。观察组和对照组GCR水平分别为3028±1402、5288±2238结合位点/淋巴细胞(t=4.99,P<0.01),CD_3的百分率分别为43.03±7.07、51.26±5.89(P<0.01)、CD_4的百分率分别为29.91±6.47、36.85±7.57(P<0.01),CD_3的百分率分别为18.56±3.68、23.41±6.82(P<0.01),CD_4/CD_3比值分别为1.63±0.31、1.65±0.41(P>0.05),IgA、IgG、IgM水平统计学处理组间无显著性差异。 结论 哮喘患儿静脉血淋巴细胞GCR水平和T细胞各亚群的百分率显著下降,表明哮喘患儿存在着细胞免疫缺陷。提示应用激活GCR的药物,对小儿哮喘的治疗可能有效。

Objectives To search for the relationship between Children' asthma and lymphocyte glucocorticoid rece-ptor (GCR). T Cell subpopulation and serum immunoglobulin. Design Case-control research. Patients and Other Panticipants Thirty-four Child patients with asthma (Observation group),Thirty-four health childreu (Control group), the sexesand ages are same as observation group. Interventions The blood of all of the patients and other participants were drawn from peripheral andfemoral vein at 8 o'clock in the morning, anticoagulated by heparin for measurement. Measurements and Main Results The GCR. T cell subpopulation and serum immunoglobulin were deter-mined by radioreceptor assay. microcytotoxicity assay and semi-automatic biochemical analysis instrumentrespectively. The GCR levels of observation group and control group are 3028±1402 and 5288±2238 com-bining site/lymphocyte respectively(t=4.99, P<0.01), thc percentages of CD. are 43.03+7.07. 51.26±5.89 respectively (P<0.01), the CD_4 are 29.91±6.47.36.85±7.57 respectively (P<0.01), the CD_8are 18.56±3.68、23.41±6.82 respectively (P<0.01), the value of CD_4/CD_8 is 1.63±0.31、1.65±0.41respectively (P>0.05).IgG. IgA and IgM levels are no significant difference between the two groups instatistics. Conclusions The GCR levels aud all of T cell subpopulation percentages are decreased obviously in thechild patients with asthma. It's indicated that the cytoimmunity is deficiency in child patients with as-thma. It's suggested that it would be effective to use the drug of activating GCR for treating children'asthma.