新型荧光试剂N-9-吖啶基氨基乙酸的合成及其光谱性质

Synthesis and Spectral Property of N-9-Acridinyl amino acetic acid

提出合成新型荧光试剂N 9 吖啶基氨基乙酸的 3种不同方法 ,其中用 9 苯氧基叮啶和氨基乙酸钠作用的间接合成法产率达 78 9%。目标产物通过红外、核磁共振等进行了结构表征 ,并对其荧光性质和紫外光谱进行了初步探讨。结果表明 ,浓度为 5 0× 10 -7mol/L的N 9 吖啶基氨基乙酸标准溶液在pH =9 0的NH4Cl/NH3 ·H2 O体系中荧光强度 (F)最大 ,F值可达 15 0 0以上 ,λex和λem分别为 2 6 8和 4 6 0nm ;在 2 0 9、2 5 3和 385nm处有明显的紫外 可见吸收峰。

A fluorescent reagent, N-9-acridinyl amino acetic acid was synthesized by three methods. The yield of the second method was 78.9%. The product was characterized by IR and 1H NMR. Its fluorescence and UV spectra were studied. A maximum fluorescence intensity(F) of the sample was recorded in NH 4Cl/NH 3·H 2O buffer system(pH9.0). The excitation wavelength and emission wavelength were 268 nm and 460 nm respectively. The UV absorption peaks of the product were at 209 nm、253 nm and 385 nm. The fluorescent reagent has a promising use for highly selective and sensitive determination of some ions or bio-macromolecules.