摘要
目的 :通过采用体细胞融合技术将西洋参基因转入胡萝卜中 ,为贵重中药、生长受地理环境等限制的中药扩大药源 ,利用杂种优势为培养适于大面积栽培且有效成分人参皂苷含量较高的优良杂交品种提供理论与实验依据。方法 :用PEG法对五加科植物西洋参与伞形科植物胡萝卜进行体细胞融合 ,通过同工酶进行初步杂种鉴定并用HPLC法测定西洋参和胡萝卜体细胞融合培养愈伤组织中人参皂苷Rb1含量。结果 :体细胞融合技术成功地获得了西洋参和胡萝卜体细胞杂交愈伤组织 ;同工酶分析是鉴定杂种的有效手段之一 ;在 10个杂交体愈伤组织中有 6个杂交体愈伤组织人参皂苷Rb1含量比未融合前西洋参愈伤组织中的含量高。
Objective: To obtain somatic hybrids between Panax quinquefolia L. and Daucus carota var.sativus Hoffm.,and provide theoretical and experimental fundament for choice of hybrid culture which adapts to great area planting and improve the content of ginseng saponins by heterosis. Methods: After somatic hybrids between Panax quinquefolia L. and Daucus carota var.sativus Hoffm. was obtained by PEG method,hybrids by Phenotype and Estenase isozyme was identified and the content of ginseng saponins Rb 1 of somatic hybridization callus was determined by HPLC method. Results: The technique of somatic hybrids of Radix quinquefolii and carota had come through. The isozyme analysis is one of efficacious means to identify hybrids.The content of ginseng saponins Rb 1 of six somatic hybridization callus in ten somatic hybridization callusare higer than those in Panax quinquefolia L. callus.
出处
《山东中医药大学学报》
2003年第6期448-451,共4页
Journal of Shandong University of Traditional Chinese Medicine
关键词
西洋参
胡萝卜
体细胞融合
杂种鉴定
人参皂苷RBL
Panax quinquefolia L.
Daucus carota var.sativus Hoffm.
somatic hybridization
hybrid identification
ginseng saponins Rb 1
