短波紫外线照射后大鼠皮肤羟脯氨酸含量的变化

L-hydroxyproline changes in rats' skin induced by ultraviolet C irradiation

目的观察大鼠皮肤伤口受不同剂量短波紫外线照射后组织中羟脯氨酸含量的变化 ,探讨紫外线照射对胶原蛋白合成的影响。方法用 3 0只Wistar雄性大鼠建立短波紫外线照射新鲜伤口的动物模型 ,在每只大鼠颈背部做 3个直径 2cm的圆形皮肤全层伤口 ,其中 2个分别接受为 15MED( 15mJ/cm2 )和 60MED( 60mJ/cm2 )短波紫外线照射 ,另 1个作为对照不接受照射 ,然后采用化学法检测伤口肉芽组织中羟脯氨酸含量的变化。结果 15MED照射伤口在照射后 2 1— 2 8d时羟脯氨酸含量高于对照 (P <0 .0 5 ) ;60MED照射伤口在 2 8d时羟脯氨酸含量显著增高 ,与 15MED照射伤口和对照之间的差异具有高度显著性意义 (P <0 .0 1)。结论短波紫外线照射能促进伤口中羟脯氨酸的合成 ,从而增加胶原含量 ,促进伤口愈合 ;60MED短波紫外线照射的作用强于 15MED。

ObjectiveTo investigate the L-hydroxyproline (L-Hyp) changes in rats' skin after exposed to different doses of ultraviolet C irradiation (UVC) and the effect of UVC radiation on collagen synthesis.MethodsAfter the animal model was set up, each male Wistar rat was made three fresh skin wounds, and three skin wounds of rats were divided into the 15 MED UVC irradiation (15mJ/cm 2), 60 MED UVC irradiation (60mJ/cm 2) and control (without UVC irradiation). Then the chemistry method was utilized to research changes of L-Hyp contents of the granulation.ResultsFrom the 21st to the 28th day after UVC irradiation, contents of L-Hyp in the 15 MED group were higher than that in controls ( P<0.05). While at the 28th day, L-Hyp level in the 60 MED group increased greatly and was higher than that in other two groups ( P<0.01).ConclusionUVC irradiation increases L-Hyp level in rat's wound skin, so it accelerates the collagen synthesis and is helpful to promote wound healing, and the effect of 60 MED dose is superior to that of 15 MED.