肿瘤坏死因子α抗体对大鼠肝脏缺血再灌注损伤的保护作用

Protective effects of tumor necrosis factor α antibody on liver ischemia reperfusion injury in rats

目的探讨肿瘤坏死因子α(TNF α)抗体对大鼠肝脏缺血再灌注损伤的保护作用。方法雄性SD大鼠 12 0只分为正常对照组 (A组 ,40只 )、缺血再灌注组 (B组 ,40只 )和TNF α抗体处理组 (C组 ,40只 ) ,于肝脏缺血 60分钟 (再灌注开始 )及再灌注1h、3h、6h、12h后 ,检测血清丙氨酸氨基转移酶 (ALT)、丙二醛 (MDA)含量及观察肝组织病理学变化。结果肝脏缺血再灌注后 ,肝脏瘀血明显 ,B组血清ALT和MDA含量均较A组明显增高 (P <0 0 1) ;与B组相比 ,C组肝组织瘀血减轻 ,血清ALT和MDA含量明显降低 (P <0 0 1)。结论TNF α抗体可以抑制大鼠肝脏缺血再灌注所致的炎症反应 ,对缺血再灌注损伤有保护作用。

ObjectiveTo study the protective effects of tumor necrosis factor α(TNF-α) antibody on liver ischemia reperfusion injury in rats. Methods120 SD rats were randomly divided into three groups, the normal control group(Group A,n=40), ischemia group(Group B,n=40) and TNF-α antibody group(Group C,n=40). After 60 minutes ischemia and followed reperfusion, the animals were killed at 0, 1h, 3h, 6h, 12h, serum concentration of alanine aminotransferase(ALT), malondialdehyde(MDA) was meassured and liver histopathology was observed. ResultsAfter ischemia reperfusion, the serum concentration of ALT and MDA in Group B significantly increased (P<0.01) ,and the hepatic congestion was obvious compared with Group A. Treatment of TNF-α antibody could significantly decrease serum concentration of ALT and MDA in Group C compared with Group B(P<0.01), and relieve hepatic congestion. ConclusionTNF-α antibody can suppress the inflammatory reaction, and protect injury induced by hepatic ischemia reperfusion in rats.