大豆黄酮对小鼠精子质量、睾丸重量及睾酮水平的影响

Effects of Daidzein on Sperm Quality,Testis Gain and Testosterone in Mice

目的 :探讨大豆黄酮对小鼠精子质量、睾丸生长和睾酮水平的影响。 方法 :给雄性小鼠饲喂 3个不同剂量的大豆黄酮 ( 5、10 0、10 0 0mg/kg日粮 ) ,2 1d后宰杀 ,睾丸称重 ,伊红丫染色确定精子存活率 ,顶体染色采用姬姆萨染色法 ,睾酮浓度采用放射免疫法测定。 结果 :5mg/kg日粮大豆黄酮能显著提高睾酮分泌水平 (P <0 .0 1) ,睾丸明显增重 (P <0 .0 5 ) ,提高了精液质量 ;10 0 0mg/kg日粮大豆黄酮抑制睾酮的分泌 (P <0 .0 1) ,精液质量变化不明显 ;10 0mg/kg日粮的大豆黄酮对精子质量等指标均无显著影响。 结论 :大豆黄酮能影响小鼠精子质量、睾丸生长及睾酮水平 。

Objective: To evaluate the effects of daidzein on sperm quality of male mice. Methods: Three different doses of daidzein were supplemented to pubertal male mice for 21 days(5 mg/kg, 100 mg/kg, 1 000 mg/kg ration). The viability of sperm was determined by eosin Y, the acrosome was observed by Wright Giemsa staining, and the testosterone was measured by radioimmunoassay. Results: Daidzein at the dose of 5 mg/kg ration significantly increased serum testosterone levels ( P < 0.01 ), prompted testis gain ( P < 0.05 ), and improved spermatozoa quality. Daidzein at dose of 1 000 mg/kg ration could inhibit the secretion of serum testosterone ( P < 0.01 ), without significant variation in spermatozoa quality. Daidzein at dose of 100 mg/kg ration did not significantly affect sperm quality and other index. Conclusions: Daidzein can affect sperm quality and in dosage dependant ways.