精子特异性乳酸脱氢酶-C4的研究进展

Advances in Researches on Lactate Dehydrogenase-C4 Isozyme

LDH C4是避孕疫苗研制中最有特征性的精子特异性抗原之一。LDH C基因在生精细胞激活而在体细胞受抑制 ,存在LDH C启动子、多种转录因子、CpG岛甲基化等多种因素在转录水平上发挥基因调节作用。针对LDH C4的高水平抗体能有效阻断受精 ,而天然LDH C4经过化学修饰后显示高度免疫原性 ,能诱导同种异体免疫性不孕。

Lactate dehydrogenase C4 isozyme has been widely studied as the sperm specific antigen in the research of the contraceptive vaccine. Developmental and testis specific expression of the LDH C gene requires mechanisms for activation in germ cells and repression in somatic cells. LDH C promoter, several transcription factors and methylation of CpG dinucleotides have been proved of governing action on LDH C gene transcription. The strategy is to induce antibodies in the female reproductive tract against LDH C antigens at a sufficient level to block fertilization. Chemically modified LDH C4 offers a potential application in the induction of infertility of homologous species in marked contrast to native LDH C4. In addition, advances are being made in researches on the male contraceptive vaccine and synthetic peptide immunocontraceptive.