中药抑抗灵治疗抗精子抗体介导的免疫性不育的实验研究Ⅰ

Chinese Traditional Medicine Yi Kang Ling to Treat Immunity Infertility Caused by Anti-sperm Antibody: the Experimental Research Ⅰ

目的 :观察中药抑抗灵对抗精子抗体 (AsAb)介导的免疫性不育的疗效。 方法 :在建立AsAb介导的免疫性不育动物模型的基础上 ,将新西兰兔随机分为 4组 ,即抑抗灵治疗组 ,强的松治疗组 ,未治疗组及阴性对照组。抑抗灵治疗组再随机分为 3组 ,即低剂量组 ,中剂量组和高剂量组。疗程均为 4 5d ,分别在治疗的第 15、30、4 5d检测原血清AsAb呈阳性的雄、雌兔血清AsAb ,计算转阴率 ;检测顺从采精雄兔的精子密度 ,活力 ,活动率及精浆AsAb。 结果 :在抑抗灵治疗的第 15、30、4 5d ,雄、雌兔血清AsAb转阴率低剂量组分别为 30 %、6 0 %、10 0 %和4 4 .4 %、77.8%、10 0 % ;中剂量组分别为 30 %、5 5 .6 %、87.5 %和 4 0 %、6 0 %、10 0 % ;高剂量组分别为 33.3%、6 6 .7%、10 0 %和 4 0 %、6 6 .7%、10 0 %。强的松治疗组雄、雌兔血清AsAb转阴率 15、30、4 5d为 30 %、6 0 %、10 0 %和 30 %、70 %、10 0 %。未经治疗的雄、雌兔AsAb的自然转阴率 15、30、4 5d分别为 0、10 %、2 0 %。经统计 ,未治疗组与抑抗灵 3个剂量组 4 5d比较 ,未治疗组与强的松治疗组 4 5d比较 ,同一剂量抑抗灵治疗 4 5d与 15d血清AsAb转阴率比较差异均有显著性 (P均 <0 .0 5 )。治疗完成后检测顺从采精雄兔精浆AsAb ,经抑抗灵低、中。

Objective: To observe the effects of Chinese traditional medicine Yi Kang Ling (YKL) on immunity infertility caused by anti sperm antibodies (AsAb). Methods: Based on the animal model of immunity infertility, seventy five pairs of Newzealand rabbits were divided into three groups: YKL treatment group (sub divided into mini , midi and maxi dosage groups), prednisone treatment group and non treatment group. Five pairs of normal rabbits were used as control. The AsAb from the rabbit serum were detected on the 15th, 30th and 45th day of treatment respectively. The sperm density, activity, the mobility and AsAb of seminal plasma from the obedient rabbits were determined. Results: Statistics showed that on the 45th day in mini and maxi YKL and prednisone treatment groups the positive serum AsAb reversing ratio reached 100%, and the seminal plasma AsAb reversing ratios were 85% in mini and maxi YKL group, 83% in midi YKL and prednisone groups, while in non treatment group the reversing ratios of the positive serum AsAb and seminal plasma AsAb were only 20% and 25% respectively. There were also remarkable differences ( P < 0.05 ) in both serum AsAb and seminal plasma AsAb on the 45th day of treatment between YKL, prednisone treatment groups and the non treatment group.Conclusions: YKL can effectively reverse the AsAb positive results, hence increasing sperm mobility and improving sperm quality.