苜蓿褐斑病抗性与几种同工酶的关系

Relationships between Pseudopeziza medicaginis resistance and several isozymes in alfalfa

以苜蓿5个品种在褐斑病抗性上存在差异的抗、感单株为材料,研究了接种病菌后苜蓿褐斑病抗性与多酚氧化酶(PPO)、过氧化物酶(POD)和超氧化物歧化酶(SOD)及其酶活性的关系。研究结果表明,褐斑病侵染后,苜蓿抗病株系和感病株系叶片中PPO、POD和SOD同工酶谱带多少和强弱存在明显的差异,即大部分抗病株系PPO、POD和SOD酶活性高于感病株系,并有新的同工酶带出现。PPO、POD和SOD在苜蓿抗褐斑病上可能起到重要作用,其同工酶谱带和酶活性的差异有可能做为鉴定苜蓿抗褐斑病的一种同工酶标记。

The relationships between resistance to Pseudopeziza medicaginis and PPO, POD, and SOD were studied in plants of 5 varieties of alfalfa (Medicago sativa). Plants within each variety with known difference in resistance to P.medicaginis were used. The results showed that, after inoculation with P.medicaginis, there were evident differences in the leaves between resistant and susceptible plants in zymograms and activities of PPO, POD, and SOD. Higher isozyme activities and more zymogram lines were detected in the resistant plants than in the susceptible plants, which suggest that the three isozymes studied may be very important in resistance of alfalfa to P.medicaginis. PPO, POD and SOD may be used as isozyme markers for identification of resistance to P.medicaginis in alfalfa.