高压电击伤家兔心肌iNOS基因异常表达研究

Study on the abnormal gene expression of inducible nitric oxide synthase(iNOS) in rabbit myocardium after high voltage electrical injury.

目的 探讨电击伤后一氧化氮 (nitricoxide ,NO)和内皮素 - 1(endothelin - 1,ET - 1)对家兔心肌损伤的影响。 方法 采用家兔高压电击伤模型 ,分别用半定量法逆转录聚合酶链反应 (RT-PCR)和血清酶法检测家兔心肌组织iNOSmRNA、ET - 1mRNA表达水平及血浆中肌酸激酶 (CK)含量。 结果 高压电击伤家兔心肌组织中iNOSmRNA 2～ 8h升高后开始下降 ,CK和ET - 1mRNA含量 2h达峰值 (CK :15 89± 75 ;ET - 1:1.16± 0 .2 5 )。

Objective To elucidate the effects of high voltage electrical injury on the mRNA expression of iNOS And endothelin-1(ET-1) in rabbit myocardium. Methods Rabbits inflicted by high voltage electrical injury were employed as the model .Reverse transcription PCR(RT-PCR) and serum enzyme measurement were used in the detection of the expression levels of iNOS mRNA and ET-1 mRNA of rabbit myocardial tissue and plasma creatine kinase(CK) content. Results The expression of iNOS mRNA in rabbit myocardial tissue after high voltage electrical injury increased at 6 to 8 postburn hours(PBH) and decreased thereafter.The plasma contents of CK and ET-1 mRNA reached their peak values at 2 PBH. Conclusion The increase of iNOS mRNA might protect myocardial tissue from electrical injury.