人外周血淋巴细胞膜电压依赖性钾通道特性分析及试分型

Property analysis of voltage-dependent potassium channel in human peripheral blood lymphocytes and its subset

目的 :研究人淋巴细胞膜电压依赖性钾〔K(v)〕通道的特性 ,为某些疾病状态下该通道特性变化提供对照。并试观察该通道是否存在不同亚型。方法 :膜片钳全细胞电流记录方法。结果 :在记录到的 39个细胞中 ,K(v)通道的激活电压为 - (40 .3± 2 .5) m V,在重复去极化状态下通道无失活 ,复极过程中通道的关闭时间为 (116 .3± 8.2 ) ms,且通道电流可被 10 mmol/ L的四乙基铵 (TEA)所阻断。结论 :人外周淋巴细胞膜可能仅具有一种〔K(v)〕通道 ,其特性与鼠 n型〔K(v)〕通道基本一致。

? Objective:To analyse the property of voltage dependent potassium 〔K(v)〕 channel in healthy people′s peripheral lymphocyte so as to contribute the control for property alteration under some pathological condition,and to try to find the new subset of this channel.Methods:Patch clamp whole cell recording technique was used.Results:In the recorded 39 cells,activated voltage of the channels was -40.3±2.5 mV.No inactivation phenomenon appeared under repeated stimulation.The closing time of the channels was 116.3+8.2 ms under the repolarization,and the current could be inhibited by 10 mmol/L TEA.Conclusion:There might be only one type of K(v) channel in human peripheral blood lymphocytes,and its properties quite resumble the type of n K(v) channel in mice. 〔