期刊文献+

不同浓度酒精灭活后的国人成骨肉瘤细胞超微结构的变化 被引量:3

Ultrastructure changes of osteosarcoma cells inactivated by alcohol in different concentrations
下载PDF
导出
摘要 目的 :探讨两种不同浓度的酒精灭活骨肉瘤细胞 (OS- 732 )后细胞内部结构变化和临床应用价值。方法 :75%和 95%酒精灭活骨肉瘤细胞 ,光镜和电镜下观察细胞形态 ,MTT法检测细胞活性。结果 :灭活后的细胞内部结构发生明显改变 ,形成不可逆性损伤。 MTT法检测证明处理后的肿瘤细胞已无生存能力。结论 :两种浓度的酒精对骨肉瘤细胞的灭活是有效的 ,为临床上骨肉瘤的保肢治疗提供良好的选择。 Objective:To study the ultrastructure changes of osteosarcoma cells(OS 732) inactivated by alcohol of two different concentrations and clinical application value.Methods:Osteosarcoma cells(OS 732) were inactivated by 75% and 95% alcohol and observed by light and electron microscope.And its viability was detected by MTT method. Results:Cell internal structure changed significantly and irreversible damage formed. MTT method proved that inactivated cells had no viability.Conclusion:These two inactivation methods were effective. Cell internal enviroment was damaged very seriously and cell was led to death. These two inactivation methods could provide choices for clinical limb protective operation. 〔
出处 《白求恩医科大学学报》 CAS CSCD 北大核心 2001年第1期49-51,共3页 Journal of Norman Bethune University of Medical Science
关键词 不同浓度 酒精 灭活 骨肉瘤 瘤细胞 超微结构 osteosarcoma alcohol inactivation
  • 引文网络
  • 相关文献

参考文献2

二级参考文献1

共引文献25

同被引文献30

  • 1李宝兴,孙世荃.骨库技术与操作规范[J].中华骨科杂志,2004,24(10):638-640. 被引量:10
  • 2杨庆诚,梅炯,蔡宣松.微波灭活骨再血管化的实验研究[J].中国肿瘤临床,2006,33(2):108-110. 被引量:5
  • 3张海波,屠重棋,段宏,裴福兴.四肢转移性骨肿瘤的手术治疗[J].华西医学,2007,22(1):80-82. 被引量:14
  • 4胡永成 卢世璧 王继芳.微波热疗在骨肿瘤保肢手术中的应用[J].中华医学杂志,1998,78(6):344-344.
  • 5Fan QY,Ma BA,Zhang MH,et al.Bone tumors of the extremitiies or pelvis treated by microwave-induced hyperthermia[J].Clin Orthop Relat Res,2003,406:165.
  • 6Lubbe AS,Bergemann C.Ultrasound therapy for malignanttumors:a conceptual assessment[J].Journal of clinical ultra-sound:JCU,1994,22(2):113.
  • 7Clarke RL&ter Haar GR.Temperature rise recorded during le-sion formation by high-intensity focused ultrasound[J].Ultra-sound in medicine&biology,1997,23(2):299.
  • 8Prat F,Chapelon,J Y,Chauffert B,et al.Cytotoxic effects of a-coustic cavitation on HT-29cells and a rat peritoneal carcinoma-tosis in vitro[J].Cancer research,1991,51(11):3024.
  • 9Harrison GH,Balcer-Kubiczek EK,Eddy HA,et al.Potentiationof chemotherapy by low-level ultrasound[J].International jour-nal of radiation biology,1991,59(6):1453.
  • 10Yang R,Reilly CR,Rescorla FJ,et al.Effects of high-intensityfocused ultrasound in the treatment of experimental neuroblasto-ma[J].Journal of pediatric surgery,1992,27(2):246.

引证文献3

二级引证文献12

相关主题

;
使用帮助 返回顶部