结肠癌组织中p53、p14基因表达的研究

STUDY OF p53,p14 GENE EXPRESSION IN COLONIC CANCER

目的 探讨结肠癌组织中 p5 3、p14基因的表达及其与结肠癌某些生物学行为的关系。 方法 经病理诊断明确的内镜活检组织标本 318例 ,其中癌组织 12 6例 ,腺瘤组织 84例 ,正常组织 10 8例。采用免疫组织化学S -P法检测三种组织中p5 3、p14的表达。 结果 p5 3在结肠癌组织中的表达明显高于正常组织 (P<0 .0 5 ) ,p14在腺瘤和癌组织中的表达低于正常结肠组织 (P <0 .0 5 ) ,p14与癌组织的临床分期及组织分化程度相关 (P >0 .0 5 ) ,p5 3与 p14的表达存在负相关性 (r =- 0 .816 9)。 结论 p5 3、p14基因的表达异常可能与结肠癌的发生发展有关 ,联合检测两者的协同表达可作为结肠癌辅助诊断的参考指标。

Objective: To study the association of p53, p14 gene expression and colonic cancer several biological behaviors.Methods:The apotosis regulatory proteins expression of p53, p14 were determined by immunohistochemical staining in colon endoscopic specimens of 108 cases of human normal colon and 84 cases of colonic adenomas and 126 cases of colonic adenocarcinomas mucosal lesion. Results:The positive staining rates of p53 in carcinomas were higher than that in normal mucosa ( P <0.05). The positive staining rates of p14 in adenomas and carcinomas were lower than that in normal mucosa ( P <0.05). The expression of p14 is related to cancer tissues differentiation and clinical stage. Their correlation analysis suggusted that there were negative correlation between p53 and p14 (r=-0.8169).Conclusions: These suggest that p53, p14 are expressed in human colonic cancer, which might contribute to various biological behaviors of the cancer. United detection of p53 and p14 might be acted asanassistant marker of colonic cancer.