核因子κB在大鼠多器官功能障碍综合征中的表达及意义

Expression of NF-KB in rats with multiple organ dysfunction syndrome

目的:探讨多器官功能障碍综合征(MODS)大鼠肝、肺、肾和小肠组织中核因子xB(NF-kB)的DNA结合蛋白和NF-kB P65蛋白的表达及意义。方法:32只雄性SD大鼠随机分成MODS组(n=16)和正常对照组(n=16),采用二次打击建立MODS大鼠模型,检测大鼠肝功能(ALT、AST)、肾功能(Cr、BUN)、动脉血氧分压(PaO2);用电泳迁移率改变试验(EMSA)检测大鼠肝、肺、肾和小肠组织中NF-kB的DNA结合活性;用Western杂交检测NF-kB P65蛋白表达。结果:衰竭组大鼠肝、肺、肾和小肠组织中NF—kB的DNA结合活性及NF—kB P65蛋白表达明显高于正常组(P<0.01);血清中ALT、BUN分别与肝、肾组织NF—kB的活性呈正相关(P<0.01)。结论:NF—kB活化在MODS的发生发展中可能起重要作用。

Objective:To investigate the expression of nuclear factor-kappa B(NF-icB) in rats with multiple organ dysfunction syndrome (MODS). Methods: Totally 32 healthy male SD rats were randomly divided into 2 groups. Sixteen rats were subjected to two-hit to establish MODS model (MODS group), and 16 rats didnot have any intervention (control group). DNA binding activity of NF-KB was measured by electrophoretic mobility shift assay (EMSA). The expression of P65 protein subunit of NF-kB was detected with Western blot. Results; DNA binding activity of NF-kB and the expression of P65 protein in different organs, including livers, lungs, kidneys and small intestines, were remarkably higher in MODS group than that in control group. Serum concentration of ALT and BUN had positive correlation with DNA binding activity of NF-KB in the liver and kidney. Conclusion:NF-kB may play a very important role in the genesis and development of MODS.