P27^(kipl)和突变型P53蛋白在甲状腺肿瘤中表达的意义

Expression of P27~kipl and mutant P53 protein in thyroid tumors

目的:研究P27kipl和突变型P53的表达与甲状腺肿瘤临床病理因素的关系,了解P27kipl和突变型P53在甲状腺肿瘤进展中的作用。方法:用免疫组化方法检测P27kipl及突变型P53在甲状腺肿瘤中的表达水平,并作相关分析。结果:P27kipl在甲状腺腺瘤中的阳性表达率(90.0％,18/20)显著高于在甲状腺癌中的表达率(60.0％,24/40),P<0.05;突变型P53在甲状腺腺瘤中不表达(0/20),而在甲状腺癌中表达率为32.5％(13/40),P<0.01。P27kipl在分化好、分期早、无淋巴结转移的甲状腺癌的阳性表达率明显高于分化差、分期晚、有淋巴结转移的甲状腺癌的阳性表达率(P<0.05,P<0.01);而突变型P53在分化差伴有淋巴结转移的甲状腺癌中表达率显著增高(P<0.01,P<0.05);P27kipl与突变型P53的阳性表达率呈显著负相关(r=-0.450,-0.414,P<0.01)。结论:P27kipl表达下调和突变型P53的表达上调可能是甲状腺肿瘤高度恶性的重要标志。

Objective:To study the expression of P27kipl and mutant P53 protein in thyroid tumors, and to understand the relationship between the biological behavior and prognosis of benign and malignant thyroid tumors. Methods: We used S-P immunohistochemical technique to study the expression of P27kipl and mutant P53 protein in 60 cases of thyroid tumors, including 40 cases of malignant tumors and 20 cases of benign tumors. Categorical data were analyzed by using X2 tests. All reported significance levels were two-tails. The software system used for statistical analysis was SPSS10. 0. Results: In benign and malignant tumors, P27kipl protein positive expression was detected in 18 of 20 benign cases (90. 0%) and 24 of 40 malignant cases (60. 0% ) respectively(P<(0. 05). The expression of P53 protein was positive in 13 of 40 malignant cases (32. 5% > P<0. 01). Low P27kipl expression in thyroid carcinomas was significantly associated with late TNM stage, high incidence of lymph node metastasis and poorly differentiated carcinomas with unfavorable pathologic features(P<0. 05, P<0. 01). An inverse correlation was observed between the expression of P27kipl and P53 protein in all thyroid tumors(r= -0. 450, -0. 414, P<0. 01). Conclusion: The down-regulation of P27kipl and up-regulation of mutant P53 protein in thyroid tumors probably promotes the progression of thyroid carcinomas and may be an indicator of high-grade malignancy.