青杨双价抗虫基因转化系统研究

Study on the transformation of Populus cathayana with the divalent insect-resistant genes

利用农杆菌介导法,对青杨进行含部分改造的BtCryAc基因(Bt基因)和慈姑蛋白酶抑制剂(API)基因A的双价抗虫基因转化,确定了适于青杨的遗传转化系统。经含Km40mg/L的生根培养基的筛选,获得了青杨转基因植株。

Explants of Populus cathayana were transformed with Agrobacterium tumefaciens LBA4404 containing the divalent insect-resistant genes. Transformation system suitable for Populus cathayana is approved. The insect-resistant plants of Populus cathayana selected on rooting medium containing Km 40 mg/L were obtained. These were the preliminary selected transformed plants.