金钱鱼凋亡相关基因SaAR对NIH-3T3细胞凋亡的影响

Effects of Scatophagus argus apoptosis-related gene on apoptosis of NIH-3T3 cells

目的观察金钱鱼凋亡相关基因SaAR(Scatophagusargusapoptosis related gene)对成纤维细胞增殖的影响。方法体外培养NIH-3T3细胞,应用脂质体将不同浓度的pcDNA3-SaAR真核表达质粒分别转染细胞并设立对照组进行比较,应用流式细胞术测定细胞凋亡率。结果SaAR基因可显著诱导NIH-3T3细胞凋亡,并呈现剂量依赖性(P<0.05)。结论SaAR基因对体外培养的血管外膜成纤维细胞凋亡有一定的诱导作用。

Objective To observe the effects of Scatophagus argus apoptosis-related (SaAR) gene transfer on the apoptosis of NIH-3T3 cells. Methods A controlled observation of SaAR gene transfer (at different concentrations) into cultured NIH-3T3 cells via lipofectin was performed, and the rate of cell apoptosis was analyzed by flow cytomertry. Results The rate of cell apoptosis was (6.37±0.0.56)% in the control group, and was (11.89±1.13)%, (18.25±1.86)% and (22.34±1.08)% respectively in pcDNA3-SaAR gene groups corresponding to pcDNA3-SaAR gene concentrations of 2, 3 and 4 μg/ml respectively. It was shown that SaAR gene significantly induced fibroblast apoptosis when the concentration was above 2 μg/ml (P<0.05), in a dose-dependent manner as compared with the control group (P<0.05). Conclusion Transient transfection of SaAR gene can significantly induce apoptosis in NIH-3T3 cells.