E-选择素第2外显子G98T基因多态性与高血压病的相关性研究

Association study of G98T polymorphisms in the exon 2 of E-selection gene with essential hypertension

目的 观察原发性高血压病(EH)患者E-选择素(E-selectin)基因第2外显子G98T多态性,并探讨高血压发病的遗传学机制。方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测155例高血压患者和160例正常对照者E-selectin基因型,生化技术测定血脂水平。结果 E-selectin基因型GG、GT频率在高血压组和对照组分别为85.2％、14.8％和92.5％、7.5％;等位基因G、T频率在高血压组和对照组分别为92.6％、7.4％和96.3％、3.7％。基因型频率和等位基因频率在高血压组和对照组比较差异均有显著性(P<0.05)。结论 E-selectin第2外显子G98T基因多态性与高血压的发病有关性,T等位基因可能是高血压发病的危险因素之一。

Objective To study the association between the G98T polymorphism in the exon 2 of E-selectin gene and essential hypertension (EH), and to explore the genetic variation in essential hypertension. Methods Genotypes of E-selectin was typed in 155 EH patients and 160 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Other plasma lipid levels were measured by biochenfical technology. Results E-selectin genotype frequencies of GG, GT were 85. 2%,14. 8% and 92. 5%,7. 5% in EH group and control group respectively. Allele frequencies of G, T were 92. 6%,7. 4% and 96. 3%, 3. 7% in EH group and control group respectively. There was significant difference in frequencies of allele and genotype in E-selectin G98T polymorphism between EH group and control group (P<0. 05). Conclusion E-selectin G98T polymorphism is associated with essential hypertension, and T allele may be a risk factor for essential hypertension in Chinese.