TNF-α与IL-2诱导人多形性腺瘤细胞凋亡

Apoptosis of salivary pleomorphic adenoma cells induced by TNF-α and IL-2 in vitro

目的 探讨肿瘤坏死因子 -α(tumornecrosisfactor-α,TNF -α)与白细胞介素Ⅱ (interleukin - 2 ,IL -2 )在体外对涎腺多形性腺瘤细胞凋亡的诱导作用。方法 取呈对数生长的第 12代人多形性腺瘤细胞 (SPA - 0 2 ) ,采用TNF -α与IL - 2单独或联合用药。应用流式细胞术检测肿瘤细胞凋亡率及细胞周期分布情况 ,利用光镜观察凋亡细胞的形态学改变。结果 流式细胞术检测发现 ,采用TNF -α 2 4h后凋亡峰开始形成 ,72h后细胞凋亡率最高。联合应用IL - 2较单独应用TNF -α凋亡率显著增高 ;光镜观察发现用药后大量多形性腺瘤细胞胞浆皱缩 ,胞核染色质固缩 ,呈大小不等的点状。结论 TNF -α可诱导体外涎腺多形性腺瘤细胞发生凋亡 ,IL - 2可增强TNF

Objective To investigate the mechanism of apoptosis of salivary pleomorphic adenoma (SPA) cells induced by tumor necrosis factor-α(TNF-α) and Interleukin-2(rhIL-2).Methods The 12th passage of SPA-02 cells were entered into this study,after treated with either TNF-α or both TNF-α and IL-2,the percentage of apoptotic cells and the changes of cell cycle were detected by the flow cytometry.The morphological changes of apoptotic cells were observed under light microscopy (LM).Results The flow cytometry showed that the typical “apototic peak” was initially formed after SPA cells were treated with TNF-α for 24h,and the percentage of apoptotic cells reached to the highest after treated with TNF-α for 72h,the percentage of apoptotic cells was higher with both TNF-α and IL-2 than that of only with TNF-α.After the cells were treated with both TNF-α and IL-2 for 72h,LM revealed many apoptotic cells with their cytoplasm shrinked.The chromatin condensed into block,and dispersed unevenly.Conclusion Apoptosis of SPA cells can be induced by TNF-α in vitro.The percentage of apoptotic cells induced by both TNF-α and IL-2 was much higher than that of only by TNF-α.