^(131)I标记的一种抗植物蛋白抗体及其片段在荷瘤小鼠体内的分布

BIODISTRIBUTION OF ^(131)I LABELLING ANTI-PLANT PROTIEN ANTIBODY AND IT′S FRAGMENT IN TUMOR BREAING MICE

目的研究13 1碘标记的抗植物蛋白抗体 (13 1I anti plantproteinantibody ,13 1I APPAb)及其片段 [13 1I F(ab′) 2 ]在荷瘤小鼠体内的生物学分布。方法氯胺 T法将13 1I标记于APPAb及F(ab′) 2 分子上。成年昆明种小鼠 ,于前肢根部皮下接种H2 2 肝癌瘤细胞使其荷瘤 ,随机分组。尾静脉注射13 1I APPAb或13 1I F(ab′) 2 185KBq(0 .2ml·每鼠 )。于注药后 12、2 4和 4 8h分别处死动物 ,取血液、心、肝、脾、肾、肌肉和肿瘤组织 ,用r计数器测量各组织放射性。结果各组织中以肿瘤组织放射性摄取率 (%ID·g-1)最高 ,不同组不同时相13 1I F(ab′) 2 的 %ID·g-1均高于13 1I APPAb ,而且13 1I F(ab′) 2 的摄取速度也较13 1I APPAb快 ,12h即达到最大值 ,13 1I APPAb则需 4 8h。肿瘤组织对正常组织的摄取比值 (T/NT)在 3～ 6之间。血液中的放射性浓度较高 ,T/NT比值也低。结论肿瘤组织中13 1I APPAb和13 1I F(ab′) 2 的摄取明显高于正常组织 ,13 1I F(ab′) 2 的摄取速度快 ,这为肿瘤的免疫诊断和免疫治疗研究提供一种新途径。

ObjectiveTo study the biodistribution of 131I labelling anti-plant protein antibody( 131I-APPAb) and it's fragment [ 131I-F(ab′) 2)]in tumor breaing mice.MethodsAPPAb and F(ab′) 2 were labelled with 131I by the chloramine-T method.The hepatocellular carcinoma(H 22) bearing Kunming mice were divided into groups. 131I-APPAb or 131I-F(ab′) 2 was injected by tail vein in mice with tumor,185KBg( 0.2ml·each),respectively.Radioactivity of different tissue (blood,heart,liver,spleen,kidney,muscle and tumor) was measured by r-counter at 12 h,24 h and 48 h after injection for biodistribution study.ResultsThe radioactivity uptake (%ID·g) of tumor was highest in all tissues.At different time points on different group,uptake of 131I-F(ab′) 2 was higher than 131I-APPAb and velocity of it's uptake also was quickly. 131I-F(ab′) 2 and 131I-APPAb has reached highest at 12 h and 48 h respectively.Tumor to normal tissue ratio of uptake (T/NT) was 3 to 6,but radioactivity concentration of blood was higher than other tissue,and tumor to blood ratio also was lower.ConclusionAccumulation of 131I-F(ab′) 2 and 131I-APPAb in tumor tissue was higher significantly than normal tissue and velocity of 131I-F(ab′) 2 uptake was quicker than 131I-APPAb.It could make a method of immunodiagnosis and immunotherapy in tumor.