毛新杨×毛白杨AFLP分子遗传图谱

An AFLP Linkage Map Using a Hybrid Population of Populus tomentosa

利用毛新杨×毛白杨的杂交群体构建了毛新杨×毛白杨的AFLP分子遗传图谱,这个群体对杨树溃疡病的抗性是正态分布。本实验使用AFLP分子标记技术对这一构图群体进行了分析,共选用了19对PstI/MseI引物和4对EcoRI/MseI引物,得到标记662个,其中3∶1标记占43 2%,异倍标记占14 5%,偏分离标记(P<0 05)占48 8%。在双亲整合的图谱中,239个标记形成22个含4个以上标记的连锁群,总图距4418cM,标记间的平均图距是18 5cM。图中还有8个重复位点和7个只与连锁群中的某一个标记连锁,但却无法加入这一连锁群的标记,这可能是由染色体的同源性造成的。此外还得到15个属于双亲共有的只有2～3个标记的小连锁群,13个父本独有的连锁群和21个母本独有的连锁群。

The map was constructed using Populus tomentosa × P.alba var. pyramidalis × P. tomentosa hybrid population in which the resistance of Dothrorella gregaria Sacc was segregated.The authors had obtained 662 markers using 19 PstI/Mse1 primer pairs and 4 EcoRI/Mse1 primer pairs,of which 43. 2% had 3∶1 in segregation,14. 5% appears to be non-parental heteroduplex,and 48. 8% was distorted.239 markers had been mapped into 22 linkage groups (each including 4 loci or more),and the integrated map was 4 418 cM in length,with an average locus interval of 18. 5 cM.Linkage group LG1 was the largest one and covered 524 cM with 25 markers.The densest linkage group was LG13 with an average interval of 6. 7 cM in length.There were duplicated loci for 8 markers,and 7 markers that linked to one of markers in a linkage group but couldn′t be mapped into that group.In addition,there were 15 minor groups harboring 2～3 markers belonged to the parents.Moreover,13 and 21 groups belonged to paternal and maternal parent,respectively.