山莨菪碱改善大鼠冠状动脉微循环及其机制的研究

Effect of anisodamine on coronary microthrombosis induced by microspheres in rats

目的 :观察山莨菪碱对大鼠冠状动脉微循环的影响 ,并探讨其可能的作用机制。方法 :通过在SD大鼠冠状动脉内注射 42 μm微粒子的方法构建冠状动脉微栓塞模型 ,将模型大鼠随机分为正常对照组 (n =6)、治疗组 (山莨菪碱 10mg·kg- 1 ·d- 1 ,腹腔注射 ,1次·d- 1 ,n =15 )和微栓塞组 (生理盐水代替 ,n =15 ) ,用非放射性彩色微粒子 (15 μm)观察术后 2 4h心肌局部血流量的变化 ,采用超声心动图评价大鼠术后第 1、3、7天的心功能变化 ,同时观察治疗组和微栓塞组间氧化应激 [指标为超氧化物歧化酶 (SOD)活力及丙二醛(MDA)水平 ]的差异。结果 :术后 3h治疗组大鼠心肌内微栓塞数量明显少于微栓塞组(P <0 .0 5 ) ,氧化应激减轻 (SOD活力比较 ,P <0 .0 5 ;MDA水平比较 ,P <0 .0 0 1) ;术后 2 4h心肌局部血流明显增加 (P <0 .0 0 1) ;第 1、3、7天大鼠的左心室射血分数分别较对照组高 13 %、3 3 %、2 7%。结论 :山莨菪碱可明显减少大鼠心肌微梗死的面积 ,增加局部心肌血流量 ,改善心功能 。

Objective To investigate role of anisodamine on the microcirculation disorder induced by microspheres. Methods 3 male Sprague-Dawley rats(weighing 250 to 300-g)were anesthetized with ketamine hydrochloride(100-mg·kg -1) given intraperitoneally and coronary microthrombosis was induced by injection of microspheres into the coronary, 10-mg·kg -1 of anisodamine was injected peritoneally. At 3 hours after operation, the oxidativestress and inflammatory reaction in the myocardium were examined along with myocardial blood flow(MBF)of the left ventricule by colored microspheres. The cardiac function was also examined at 1st ,3rd and 7th day after operation.Results The microthrombosis was successfully induced and anisodamine could significantly attenuate the oxidative stress and inflammatory reaction in the myocardium.The MBF in the left ventricule markedly increased from (2.34±1.23) to (3.83±1.51)ml(g·min) -1,P<0.001 compared with that of control.On 1st ,3rd and 7th day,the ejection fraction of the left ventricule was significantly increased compared with that of control(P<0.001). Conclusion The present study shows that the anisodamine could protect microcirculation from the disorder induced by microspheres possibly due to its antioxidative effects.