川芎嗪对哮喘大鼠气道壁平滑肌增殖及TGF-β_1表达的影响

Effect of ligustrazine on airway smooth muscle proliferation and TGF-β_1 expression in asthmatic rats

目的 :观察川芎嗪对哮喘大鼠气道壁平滑肌增殖及转化生长因子 (TGF β1 )表达的影响。方法 :3 2只SD大鼠均分为正常对照组、以卵蛋白致敏制备的哮喘模型组以及给予不同剂量川芎嗪进行干预的小剂量、大剂量川芎嗪干预组共 4组 ,采用免疫组织化学和计算机图像分析方法测定各组大鼠气道壁TGF β1 含量、平滑肌层厚度及内外径。结果 :模型组气道壁TGF β1 含量和平滑肌层厚度较正常组均显著增加 (均为P <0 .0 0 1) ,气道内外径比值较正常组减小 (P <0 .0 0 1)。两种剂量川芎嗪干预组平滑肌层厚度均较模型组变薄 (均为P <0 .0 0 1) ,TGF β1 含量均较模型组减低 (均为P <0 .0 0 1) ,气道内外径比值均高于模型组 (均为P <0 .0 0 1) ,两种剂量川芎嗪干预组间平滑肌厚度无差别 (P >0 .0 5 ) ,大剂量组对TGF β1 表达抑制较小剂量组强 (P <0 .0 1)。气道壁平滑肌层厚度与TGF β1 表达呈正相关 (rs=0 .5 878,P <0 .0 1)。结论 :川芎嗪能减轻哮喘大鼠气道壁平滑肌层的增厚并抑制TGF β1

Objective To observe the effect of ligustrazine on the proliferation of airway smooth muscle and the expression of transforming growth factor-β 1 (TGF-β 1) in asthmatic rats. Methods 32 SD rats were randomly divided equally into 4 groups:normal group, model group,low-dose ligustrazine group and high-dose ligustrazine group. The asthmatic rat model was established by repeated inhalation of ovalbulium. The changes of TGF-β 1 content in the wall of airway and the thickness of airway smooth muscle layer, inner and outer diameter were measured by the computerized image analysis system. Results The wall contents of TGF-β 1 and the thickness of airway smooth muscle layer were significantly higher ( P< 0.001) in the model group than that in the normal group, while the ratio of inner diameter to outer diameter were lower ( P< 0.001) .The contents of TGF-β 1 in both low-dose and high-dose ligustrazine group, were lower(P<0.001)than that in the model group, and all close to the normal group ( P> 0.05), the thickness of airway smooth muscle layer were lower (P<0.001) too, but the ratio of inner diameter to outer diameter were higher (P<0.001). A close correlation between the thickness of airway smooth muscle layer and contents of TGF-β 1 was demonstrated(r s=0.587-8, P<0.01).Conclusion Ligustrazine could inhibit the airway remodeling by the way of reducing the proliferation of airway smooth muscle and the expression of TGF-β 1 in asthmatic.