胃癌组织CD_(44)v9和MMP-2基因的表达

Relation between CD_(44)v_9, MMP-2 and tumor invasion and metastasis in gastric cancer

目的:研究CD_(44)v9和MMP-2在胃癌和癌旁组织中的表达,探讨胃癌侵袭与转移的可能机制。方法:采用RT-PCR方法分别检测40例胃癌及癌旁组织CD_(44)v9及MMP-2的阳性表达情况。结果:40例胃癌组织中CD_(44)v9和MMP-2的阳性表达率分别为75％和82.5％,明显高于癌旁组织的35％和48.5％,二者比较差异均有显著性(x^2=12.929;x^2=10.769.P均≤0.001)。CD_(44)v9和MMP-2表达与肿瘤的大小、分化高低、浸润深度及临床分期有关,合并有淋巴结转移的17例胃癌患者CD_(44)v9和MMP-2表达率明显高于不伴有淋巴结转移的胃癌患者(P均<0.05),CD_(44)v9和MMP-2的表达与胃癌有相关性(r=0.6,P<0.001)。结论:CD_(44)v9和MMP-2与胃癌侵袭和转移性有关,可作为预测肿瘤转移潜能的指标。

AIM: To study the expression of matrix metalloproteinase CD_(44)V9, matrix metalloproteinase-2 (MMP-2) in gastric cancer and the corresponding adjacent normal tissues, to investigate the possible mechanism of tumor invasion and metastasis in gastric cancer. METHODS: TUmor tissues and adjacent normal tissues in 40 cases of gastric cancer were detected by using reverse tenscriptase-polymerase chain reaction (RT-PCR) for the expression of CD_(44)v9, MMP-2. RESULTS: The expression of CD_(44)v9, MMP-2 in tumor tissues were higher than that in adjacent normal tissues, respectively (x^2=12.929, X^2=10.769, P≤0.001). The expression was related to tumor size, degree of differentiation, clinical staging. The expression of CD_(44)v9, MMP-2 mRNA in 17 gastric cancers with lymph node metastasis were higher than that in gastric cancer without lymph node metastasis (P<0.05). The expression of CD_(44)v9, MMP-2 correlated highly with gastric cancer (r = 0.6, P<0.001). C0NCLUSION: CD_(44)v9, MMP-2 were related to tumor invasion and metastasis in gastric cancer, and could be used as important indexes to predict invasion and metastasis of gastric cancer.