两种方法检测新生儿肺炎沙眼衣原体感染

COMPARISON OF TWO METHODS OF DETECTION CHLAMYDIA TRACHOMATIS IN NEONATAL PNEUMONIA: TISSUE CULTURE AND POLYMERASE CHAIN REACTION

应用改良McCoy细胞培养法和聚合酶链反应(PCR)检测新生儿肺炎患儿沙眼衣原体(CT)感染。两种方法检测328例新生儿感染性肺炎患儿鼻咽拭子,共证实CT肺炎67例,阳性率为20.4％,以“扩大金标准”来判断结果,PCR的敏感性和特异性分别为98.5％和100％,而培养的敏感性仅89.6％;聚乙二醇(polyethylene glycol,PEG)可提高培养的敏感性。表明CT是新生儿肺炎的重要病原菌。PCR检测CT更敏感、快速。

To investigate the prevalence of neonatal pneumonia caused by Chlamydia trachomatis(CT) and to evaluated tissue culture and PCR in detecting CT infection. 328 cases of neonatal pneumonia were studied by modified McCoy cell culture and polymerase chain reaction(PCR) by nasopharvngeal s\vabs. Among them, the prevalence of CT infection was 20.4% (67/328). Compared to expanded gold Standard of CT in detecting CT infection, the sensitivity and the specificity of PCR were 98.5% and 100% respectively. CT is an important pathogen of neonatal pneumonia, the PCR appears to be equally specific and more sensitive than McCoy celi culture (sensitivity was 89.6%) for the detection of CT from nasopharyngeal specimens. Using of polyethylene glycol could improve the sensitivity of detecting CT in tissue cuilture.