Y染色体丢失与其它染色体畸变在急性髓性白血病中的意义

The Significance of Y Chromosome Loss and Other Chromosome Aberration in Acute Myelogenous Leukemia

为了研究Y染色体丢失与染色体畸变在急性髓性白血病中的临床意义,采用了细胞遗传学方法及Y染色体荧光原位杂交技术对29例骨髓和血液标本进行了检测,并观察了两种方法所得结果与诊断、疾病进展和预后的关系。结果发现:①细胞遗传学方法检测染色体异常率为83％;②原位杂交技术检测Y染色体的丢失比细胞遗传学方法更敏感;③染色体畸变及Y染色体丢失对疾病诊断、病情进展及预后有价值;④两种方法均显示:处于非缓解期的患者Y染色体丢失率高,反之则低。以上结果为指导急性髓性白血病的治疗提供了有用的信息。

To investigate the significance of chromosome abnormalities in acute myelogenous leukemia (AML), samples of bone marrow and peripheral blood from 29 AML cases were analyzed by EB synchronization method and the Y-chromosome fluorescence in situ hybridization (FISH). Furthermore, the association of chromosome abnormalities with diagnosis, progress and prognosis of the disease was observed. It was found that the positive rate of chromosome abnormalities with cytogenetic method was 83 percent. With either EB synchronization or Y-FISH, the higher proportion of Y chromosome loss was observed in relapse phase. However, the lower proportion would occur in remission phase. The sensitivity of Y-FISH was higher than that of cytogenetic method in detecting chromosome abnormalities. The results from Y-FISH could provide important clues and values to the suitable diagnosis and treatment and prediction of prognosis.