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Expression of Bcl-2 induced by IGF-1 in NSCs and protective effect of Bcl-2 on NSCs deprived of browth factors 被引量：1

Expression of Bcl-2 induced by IGF-1 in NSCs and protective effect of Bcl-2 on NSCs deprived of browth factors
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摘要 Objective: To study the effect of IGF-1 on the expression of Bcl-2 of NSCs and the protective effect of Bcl-2 on the NSCs deprived of browth factors. Methods: The neural stem cells wereisolated from hippocampi of newborn rats and were cultured by using serum-free medium containing EGF 20ng/ml and bFGF 20ng/ml. Expression of Bcl-2 and nestin antigen were observed by using immunocytochemistry method, MTT method was used to analyse the vitality change of NSCs cultured with IGF-1 and without IGF-1 after withdrawing growth factors. Results: After cultured 7d with IGF-1,the cells were almost positive expression of nestin antigen, and (21.3±0.4)% of all NSCs were Bcl-2 positive cells, furthermore, these cells had a larger survival term against withdrawing growth factors than that cultured without IGF-1.Conclusion: IGF-1 could promote the expression of Bcl-2 in NSCs without affecting the characters of positive expression of nestin antigen, moreover, could increase the self-protection ability through elevating expression of Bcl-2 against deprivation of growth factors in the NSCs. Objective: To study the effect of IGF-1 on the expression of Bcl-2 of NSCs and the protective effect of Bcl-2 on the NSCs deprived of browth factors. Methods: The neural stem cells wereisolated from hippocampi of newborn rats and were cultured by using serum-free medium containing EGF 20ng/ml and bFGF 20ng/ml. Expression of Bcl-2 and nestin antigen were observed by using immunocytochemistry method, MTT method was used to analyse the vitality change of NSCs cultured with IGF-1 and without IGF-1 after withdrawing growth factors. Results: After cultured 7d with IGF-1,the cells were almost positive expression of nestin antigen, and (21.3±0.4)% of all NSCs were Bcl-2 positive cells, furthermore, these cells had a larger survival term against withdrawing growth factors than that cultured without IGF-1.Conclusion: IGF-1 could promote the expression of Bcl-2 in NSCs without affecting the characters of positive expression of nestin antigen, moreover, could increase the self-protection ability through elevating expression of Bcl-2 against deprivation of growth factors in the NSCs.
作者 ZHU Xiao-feng XU Zhong-ye ZHANG Xiao-mei ZHOU Hou-lun
机构地区 SecondAffiliatedHospital.JiamusiUniversity TongjiMedicalCollege
出处 《黑龙江医药科学》 2003年第5期7-9,共3页 Heilongjiang Medicine and Pharmacy
基金 Supported by National Natural Science Fundation of China( 30 1 70 963)
关键词 神经干细胞 中枢神经损伤 细胞因子 诱导 IGF-1 BCL-2 neural stem cells IGF-1 Bcl-2 protection
分类号 R338.2 [医药卫生—人体生理学]
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参考文献8

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同被引文献9

  • 1张小东,贾延劼,赵立波,谢鹏.神经干细胞的微环境[J].中国临床康复,2006,10(21):132-134. 被引量：5
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黑龙江医药科学
2003年 第5期

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