神经干细胞移植对Alzheimer病大鼠脑形态及动物行为学影响

The brain structure and behavior changes of Alzheimer disease rat after neural stem cell implantation

目的 :观察移植入 Alzheimer病 (AD)大鼠脑内的神经干细胞存活、分化及功能。方法 :由新生大鼠海马分离培养神经干细胞 ,采用切断穹窿海马伞的方法制作 AD大鼠模型 ,模型建立 8～ 10 d后行神经干细胞移植。移植 1个月后 ,通过暗回避试验检测大鼠的学习记忆能力 ,应用尼氏染色 ,乙酰胆碱酯酶 (Ach E)染色观察体内移植神经干细胞的存活 ,分化以及 AD大鼠 Ach E纤维密度的变化。结果 :神经干细胞在额叶和海马都能够存活 ,分化成神经元 ,可与宿主建立突触联系 ,在海马区移植神经干细胞的生长优于额叶。与对照组相比 ,接受神经干细胞移植鼠的暗回避潜伏期变长 (P <0 .0 5 ) ,探索次数减少(P<0 .0 5 ) ,海马 Ach E纤维密度增加 (P <0 .0 5 )。结论 :神经干细胞能够在 AD大鼠额叶、海马存活、分化 ,并可导致 Ach E纤维密度增加 ,AD大鼠学习。

Objective:TO investigate the survival,differentitation and function of transplanted neural stem cells(NSCs) in the brain of Alzheimer disease(AD) model rats.Methods:The NSCs from hippocampi of neonatal Wistar rats were cultured,the AD model was made by cutting the fibira-fornix unilaterally by using Wistar rat. Ten days after surgery operation,the NSCs were implanted into the hippocampi and frontals of AD model rats.One month after transplantation,the memory and learning abilities of the AD rat were dectected by the passive aviodence testing.Nissl and Acety cholinesterase (AchE) histochemical stainings were used to observe NSC survival,differentitation and the AchE fiber density.Results: Compared with the control group,the NSCs transplantation rats showed the escape latency time increased (P<0.05),the grope time decreased (P<0.05),the AchE fiber density increased (P<0.05).the NSCs implanted had more benefit growed in the happocumpus than the frontal.The neurons after differentiation may established connection with brain tissue.Conclusion:The NSCs may survive,differentiate and function after implantation to the hippocampus and frontal of the AD rat.