axl基因在骨髓增生异常综合征患者外周血细胞的表达

Study on the expression of axl gene in the peripheral blood cells of patients with myelodysplastic syndrome

为研究axl基因在骨髓增生异常综合征(MDS)及其它几种血液病患者外周血单个核细胞中的表达,用RT-PCR方法测定细胞中axl基因mRNA的表达情况。结果表明:①10/11例(91%)MDS患者和1/2例AML-M_3型患者有axl基因表达。2例再生障碍性贫血患者、2例球形红细胞增多症患者、2例正常人均无axl基因表达。②FAB分型与axl基因表达关系:5例RA有表达,其中强表达2例,弱表达3例;4例RAEB均有表达,其中强表达1例,弱表达3例;1例RAEB-T为强表达。以上结果说明,axl基因在MDS患者中有很高的表达率,提示其可能作为MDS患者的诊断指标之一。

In order to study the expression of axl gene in the peripheral blood mononuclear cells of MDS and several kinds of hematologic disorders, the mRNA of axl was analyzed by RT-PCR method. The results were as follows: 1. 10 cases from 11 cases of MDS (91 %) had positive axl expression, one of two cases of AML-M3 was positive as well, while 2 cases with aplastic anemia, 2 cases with spherocytosis and 2 in normal control group were negative. 2. The relationship of FAB subtype and the axl expression; 2 cases from 5 cases of RA had strong expression and 3 cases had weak expression; 1 cases in 4 cases of RAEB showed strong expression and 3 cases had weak expression, 1 case of RAEB-T showed strongly positive expression. These results suggested that the high axl expression in patients with MDS may be one of the diagnostic indications.