细胞因子在CD34阳性骨髓细胞中的表达(英文)

Cytokine mRNA expression of normal CD34 positive bone marrow cells

CD34^+细胞包含有造血干/祖细胞,而造血干/祖细胞能被IL-3,GM-SCF,G-CSF等诱导分化与增殖。正常的CD34^+细胞是否参与一些细胞因子的产生尚未被阐明。我们采用逆转录-聚合酶链反应分析了经流式细胞仪分选出的正常骨髓CD34^+细胞IL-1β,IL-3,IL-4,IL-6,GM-CSF,TNF-α,TNF-β及c-kit基因的表达,并且分析了重组IL-1β,IL-3,IL-7,GM-CSF,PIXY321(IL-3/GM-CSF融合蛋白)及干细胞因子(SCF)对这些细胞因子的调节作用。结果发现新分选出的CD34^+细胞表达较高水平的IL-1β,c-kit mRNA及低水平的IL-3,TNF-α,TNF-βmRNA。经外源性的细胞因子刺激后,IL-1β,TNF-α及TNF-βmRNA均有不同程度的上调,唯IL-7能使GM-CSF mRNA的表达变为阳性,IL-7亦能显著增强IL-6基因的表达。所有这些细胞因子对c-kit及IL-3基因表达均无明显作用。

CD34 + celis contain hemopoietic stem progenitor celis, vvhich can be induced to differentiate and proli-ferate by interleukine-3 (IL-3), granulocvte-macrophage colonj'-stimulating factor (GM-CSF) and granulocyte-CSF (G-CSF) etc. Whether normai CD34+ celis participate in the production of any cytokines remains uncertain. We inves-tigated IL-lp, IL-3, IL-6, IL-4, GM-CSF, TNF-a, TNF-P and c-kit gene expression by using reverse transcription-polvmerase chain reaction {RT-PCR) in flow cvtometric sorted normai bone marrow CD34 + celis. We also analyzed the effects of recombinant IL-1P, IL-3, IL-7, GM-CSF, PIXY-321 (fusion protein IL-3/GM-CSF) and stem celi factor (SCF) on the regulation of these cvtokines. It was found that freshly sorted CD34 + celis have high Ievels of IL-1P, c-kit mRNA and low Ievels of IL-3, TNF-a and TNF-P mRNA. After stimnlating with exogenous cvtokines, the Ievels of IL-1P, TNF-a and TNF-p mRNA were upregulated by all stimuli to various extent. GM-CSF mRNA became positive only under the effect of IL-7 stimulation. IL-7 also significantly enhanced IL-6 gene expression. None of these factors have a significant effect on c-kit or IL-3 transcription.