混合脐血血浆对脐血CD34^+细胞体外扩增的作用

Effect of mixed umbilical cord blood plasma on ex vivo expansion of CD34^+ cells from umbilical cord blood

采用两步法分离出脐血CD34^+细胞,比较研究了混合脐血血浆联合IL-3,IL-6,GM-CSF,Epo 4种中、晚期造血因子和单纯的4种造血因子情况下脐血CD34^+细胞的体外扩增。结果表明,混合脐血血浆联合造血因子对粒-巨噬细胞集落形成单位(granulocyte-macrophage colony-forming unit,CFU-GM),红系爆式集落形成单位(burst-forming unit of erythriod,BFU-E),混合集落形成单位(minxed colony-forming unit,CFU-mix)3种集落的扩增效果明显优于单纯的4种造血因子联合组,差异具有统计学意义(P<0.01),但单纯混合脐血血浆扩增效果较差。脐血造血细胞扩增对子成人脐血移植有重要意义。上述结果提示,混合脐血血浆的扩增成功,可代替或弥补早期造血生长因子的作用,用于脐血造血细胞的体外扩增。

Using two-step-adhering method, we separated CD34 + cells from human umbilical cord blood and compared the expanding effect of CD34+ cells from mixed umbilical cord blood plasma combined with IL-3, IL-6, GM-CSF, Epo and pure combination of IL-3, IL-6, GM-CSF, Epo. The results indicated that the expanding effect of mixed umbilical cord blood plasma combined with four kinds of hematopoietic growth factors on CFU-GM, BFU-E, CFU-mix was more superior than that of pure combination of four kinds of hematopoietic growth factors. The difference has statistical value (P<0.01), but the expanding effect of pure mixed umbilical cord blood plasma is poor. In vitro expansion is important to adult in umbilical cord blood transplantation. The successful expansion of CD34 + cells from mixed umbilical cord blood plasma showed that mixed umbilical cord blood plasma may replace or compensate the effect of earlier hematopoietic growth factors in vitro expansion.