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An efficient culture procedure for micro-propagation of rice regeneration plants

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摘要 In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature seeds of an indica rice lineG67 by culturing the husked and disinfectedseeds on agar medium,consisting of Nbasalelements,3% sucrose,1000mg/L proline,and 32mg/L 2,4-D.After one month's in- duction culture and 3 wk subculture,compactand nodular calli were picked out and trans- ferred into liquid medium for suspension cul-tures.The liquid medium contained the same
出处 《Chinese Rice Research Newsletter》 1999年第3期7-8,共2页 中国水稻研究通报(英文版)
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