原位末端标记检测食管癌凋亡的临床病理学意义

In situ end labeling detection for apoptosis in esophageal cancer and clinicopathologic significance

目的:通过对食管癌组织中细胞凋亡的原位观察,评价其临床病理学意义.方法:采用原位末端标记法检测30例食管癌石腊组织切片.结果:在确诊的30例食管癌病人中,凋亡发生率为93.3%,凋亡指数为10.07±13.11,凋亡指数高低与肿瘤病理分级及淋巴结转移明显相关(P<0.01).结论:在食管癌组织中,细胞凋亡是一种自发现象,其发生情况各有不同,可作为评价预后及指导综合治疗的研究指标之一.

Objective: This study was undertaken to determine the expression of apoptosis in esophageal cancer and to evaluate the clinicopathologic significance. Methods: A series of 30 cases with esophageal cancer was analysed for the expression of apoptosis by using the in In situ end labeling method in tissue sections. The number of apoptotic cell per 10 low-power microscopic fields was taken as the apoptotic index. Results: The in situ end labeling allowed a precise evalvation of the expression of apoptosis. Apoptosis oc-cured in 93.3 % of esophageal cancer patients, and apoptotic index was 10.07±13.11. Higher apoptotic index was related to lymph node metastasis ( P < 0.01), and poorly differentiated histopathologic grade (P < 0.01) . No singnificant differences were observed in apoptosis index in local tumor invades. Conclusion: The apoptosis was a spontaneous phenomenon in esophageal cancer tissue, and the expression was different from each other. Further analysis was needed to clarify the relationship apoptosis and prognosis, especially the response to adjevant therapy.