用微卫星标记鉴定中国小麦品种中Rht8矮秆基因的分布

Rht8 Dwarf Gene Distribution in Chinese Wheats Identified by Microsatellite Marker

利用微卫星Xgwm2 6 1标记对中国小麦主产区近 30年小麦主栽品种进行Rht8矮秆基因的鉴定 ,同时进行系谱分析加以验证 ,结果表明 :就全国范围而言 ,约 4 2 .3%的品种含有Rht8,但不同生态区的分布频率不同 ;结合赤霉酸(GA3)反应实验 ,约 2 0 .6 %的品种同时含有Rht8和对GA3不敏感矮秆基因。根据系谱分析 ,中国小麦品种Rht8的供体品种主要是来自意大利的阿夫 (Funo)、中农 2 8(VillaGlori)、郑引 1号 (St14 72 5 0 6 )、郑引 4号 (St2 4 2 2 4 6 4 )等和前苏联的无芒 1号 (Bezostaya 1)。洛类系统在中国作为抗源利用的同时 ,很可能也是Rht8的重要供体。

Wheat microsatellite Xgwm261 was used to identify Rht 8 dwarf gene in main Chinese wheat varieties released in the last 30 years. The results, supplemented by pedigree analysis, showed that 42.3% of Chinese wheat varieties contained Rht 8, but its frequency differed in different ecological zones. Combined with the results of GA 3 test, it indicated that 20.6% of Chinese wheat varieties carried both Rht 8 and GA 3 insensitive dwarf genes. For most Chinese wheat varieties, their Rht 8 could be traced back to Funo, Villa Glory, St1472/506 and St2422/464 introduced from Italy, and Bezostaya1 from Russia. 1B/1R varieties, such as Avrora, Lovrin 10, Kavkas, Predgornia 2, etc, once widely used as the parents resistant to rust and powdery mildew in China, could also be the donors of Rht 8.