高效液相色谱法测定益宫散结口服液中芍药苷的含量

Content Determination of Paconiflorin in Yigongsanjie Oral Liquid by HPLC

目的 :应用高效液相色谱法对益宫散结口服液进行含量测定。方法 :选用HypersilODS 2分析柱 (2 5 0mm×4 .6mm ,5 μm) ,乙腈 - 0 .0 5mol·L-1磷酸二氢钾 (13∶87)为流动相 ,检测波长为 2 30nm ,流速 1.0ml·min-1。结果 :线性范围为1.12～ 13.4 4 μg(r=0 .9997) ,平均回收率为 97.7% ,RSD为 1.1%。结论 :本法简便、灵敏、准确。

Objective: To establish HPLC method for the content determination of paconiflorin in Yigongsanjie oral liquid.Method: Hypersil ODS-2 column(150 mm× 4.6 mm,10 μm)was used as stationary phase,the mobil phase consisted of ethanol- 0.05 mmol KH 2PO 4(13∶87),detecting wavelength was at 230 nm.Result: The linear range of paconiflorin was between 1.12- 13.44 μg(r= 0.999 7). A average recovery was 97.7%,RSD was 1.1%.Conclusion: The methods is simple,rapid and accurate.