HPLC法同时测定血清中苯巴比妥、苯妥英和卡马西平的浓度

Simultaneous Determination of Phenobarbital,Phenytoin and Carbamazepinein Serum by HPLC

目的 :建立快速测定血清中苯巴比妥、苯妥英和卡马西平浓度的方法。方法 :色谱柱DiamonsilC18(4 .6mm× 2 5 0mm ,5 μm) ,流动相甲醇∶水∶乙腈 (4 0∶4 5∶15 ) ,流速1.2ml·min-1,检测波长 2 4 0nm ,内标硝西泮 ,柱温 4 0℃。结果 :提取回收率为 84 .4～ 89.2 % ,日内和日间RSD均 <7.9% ;标准曲线相关性良好。对 10 4例患者作血清浓度监测 ,临床效果满意。结论 :本方法可作为苯巴比妥、苯妥英和卡马西平的血药浓度监测的常规方法。

Objective: The concentrations of phenobarbital,phenytoin and carbamazepine in serum were determined simultaneously by RP-HPLC.Method: The separation was performed on DiamonsilC 18( 4.6 mm×250 mm,5 μm)column with methanol-water-acetonitrile(40∶45∶15) as mobile phase. Nitrazepam was used as internal standard. The flow rate was 1.2 ml/min .The wavelength for detection was 240 nm.Result: The extraction recovery rates were between 84.4% and 89.2%. The intra-day and intre-day were less than 7.9%.The calibrations curve were good linearity.Conclusion: This method is accuate, rapid and simple. It can be used as a roution method by TDM.