小鼠胚泡着床障碍模型的建立

Establishment of mice model with embryo implantation dysfunction

目的 建立小鼠胚泡着床障碍动物模型。方法 ①将成年雌性昆明小鼠交配后随机分为对照组和实验组 ,于妊娠d 4实验组于皮下注射不同浓度的米非司酮 ,于妊娠d 8观察小鼠胚泡着床率及平均着床胚泡数 ,筛选最佳造模剂量。②选用 0 0 8mg·只 -1米非司酮造模 ,采用放免法检测对照组和模型组的血清及子宫组织雌二醇、孕酮的含量 ,利用光镜和电镜观察子宫内膜及卵巢的形态结构。结果 ①当米非司酮的剂量达 0 0 8mg·只 -1以上时 ,着床率和平均着床胚泡数均降低。②与对照组相比 ,模型组子宫内膜的发育明显受抑制 ,而血清及子宫组织的雌二醇和孕酮的含量、卵巢的形态结构均无改变。结论 于妊娠d 4皮下注射米非司酮 0 0 8mg·只 -1,可复制机制明确。

AIM To establish mice model of embryo implantation dysfunction. METHODS ① Sexually mature virgin, Kunming mice were randomly assigned to control and experiment groups postcoitally. The experimental mice on day 4 of pregnancy were injected subcutaneously with mifepristone diluted in propylene glycol. All the animals were sacrificed on day 8 of pregnancy and their uterine horns were examined for the presence of implanted embryos. ② Estradiol and progesterone concentrations in serum and endometrium tissue homogenate were measured by radioimmunoassay. The structure of endometrium and ovary was observed by light-microscope and the ultrastructure of endometrium, inspected by electron microscope. RESULTS ①Compared with the control group,implantation rates and average embryo number significently decreased,when mifepristone was administered at more than 0.08 mg·mouse -1. ②Compared with control group, the estradiol and progesterone concentrations in serum and endometrium tissue homogenate of model mice were not significantly changed. The development of endometrium was inhibited, while the ovaries were not affected. CONCLUSION Being injected subcutaneously with mifepristone at 0.08 mg·mouse -1 on day 4 of pregnancy might duplicate an ideal mice model of embryo implantation dysfunction.