HPLC法测定人血清中多索茶碱浓度

Determination of doxofylline concentration in human serum by HPLC

目的 :建立测定人血清中多索茶碱浓度的RP HPLC法。方法 :以Techsphere ODS色谱柱为分析柱 ,流动相 :甲醇 水( 312∶4 88,V/V) ,流速为 0 8mL·min-1,检测波长为 2 73nm ,柱温为室温 ;血清样品经甲醇沉淀蛋白离心后进行色谱分析。结果 :本法在 0 75～ 2 0 0 0mg·L-1间线性关系良好 (r=0 9999) ,平均回收率为 99 2 1% ,日内及日间RSD均 <5 % ,最低检测血清浓度为 0 2 5mg·L-1。结论 :该方法快速、准确 。

AIM:To establish a method for determining the concentration of doxofylline in human serum by reversed phase HPLC METHODS:A Techsphere ODS colum was used with a mobile phase of methanol water (312∶ 488, V/V),flow rate was 0 8 mL·min -1 ,the detecting wavelength was 273 nm Serum sample was used for chromatography after protein precipitation and centrifuging RESULTS:The peak area of doxofylline against doxofylline concentration was linear with a correlation coefficicent of 0 999 9 over the range of 0 75 20 00 mg·L -1 The minimal detectable concentration of this method was 0 25 mg·L -1 The average recovery was 99 21%,and the RSD within day and between day were less than 5%,respectively CONCLUSION:This method is simple,accurate and precise. It is usable for clinical pharmacokinetic studies and monitoring of doxofylline in human serum