山东省PRRS血清学调查及PRRSV分离株ORF3-7基因的克隆及序列分析

Serological investigation of PRRS in Shandong,cloning and sequencing of ORF3-7s of PRRSV isolate

应用荧光抗体技术对山东省猪场PRRS的流行情况进行了血清学调查并分离到一株PRRSV。用 5对引物分别扩增了PRRSV山东分离株ORF3_ORF7,将其克隆入PMDl8_TVector并进行了序列测定。结果表明 ,山东分离株与美洲疫苗株ATCCVR_2 332 0RF3_ORF7各读码框核苷酸同源性分别为 98%、99%、99%、10 0 % ,氨基酸同源性分别为97%、98%、98%、98%、10 0 %。

A PRRSV strain was isolated from serum-positlye pigs identified by IFA.ORF3-7S Of PRRSV Shandong strain were cloned into PMD18-T vector and sequenced respectively.The results showed that the identities of nucleic acid of ORF3-7s between PRRSV Shandong isolate and VR-2332 were 98 %,99 %,99 %,99 % and 100 %,the identitles of deduced amino acid sequence were 97 %、98 %、98 %、98 %、100 %respectively.