鹅细小病毒不同毒株VP3基因的序列分析比较

Comparing and analyzing of the VP3 gene sequence of different goose parvovirus strains

分别将GPV 4个分离株通过PCR技术 ,从病毒基因组DNA中扩增出病毒衣壳蛋白VP3完整基因片段 ,并与PMD18_T质粒载体连接 ,转化到感受态大肠秆菌TG1中 ,提取重组质粒经PCR鉴定和酶切鉴定后 ,对插入片段进行序列测定及分析。结果表明 :VP3基因全长 16 0 5bp ,编码 5 34个氨基酸。不同毒株主要结构蛋白VP3基因同源性较高(95 .6 4 %～ 99.81% )。但强弱毒株间也存在一定差异。

VP3 gene of different Goose parvovirus strains were amplified from the DNA by Polymerase Chain Reaction (PCR), the Vp3 genes were cloned into PMD 18-T vector respectively. The recombinant plasmid were identified with restriction endonuclease analysis and PCR, and then sequenced.The result of sequence analysis showed that VP3 gene had 1 605 bp and included a complete open reading frame which encoding a protein of 534 amino acids,and all the four strains of GPV shared highly common identity(95.64 %～99.81 %),but there is also some difference between highly lethal strains and mild strains.