实验性糖尿病对大鼠胸腺巨噬细胞和交错突细胞的影响

The influence of experimental diabetes on the macrophages and interdigitaing cells in rat thymus

目的 探讨糖尿病对大鼠胸腺巨噬细胞和交错突细胞的影响及其在胸腺退化中的作用。方法 采用四氧嘧啶型糖尿病动物模型 ,应用酶组织化学、免疫组织化学和透射电镜方法 ,对糖尿病大鼠胸腺巨噬细胞和交错突细胞进行定性和半定量分析及形态学观察。结果 在糖尿病大鼠胸腺中 ,巨噬细胞呈ACP、NSE强阳性组化反应及呈Ly、AACT强阳性免疫反应 ,细胞数量明显增多 ,大量聚集于皮质和皮髓质交界区 ,皮质尤为明显。交错突细胞呈S -10 0蛋白强阳性免疫反应 ,细胞数量则明显减少 ,主要分布于髓质和皮髓质交界区。电镜观察皮质巨噬细胞胞质含有被吞噬的凋亡细胞或小体 ,而皮髓质交界区巨噬细胞胞质则含有囊泡和颗粒。结论 糖尿病大鼠胸腺巨噬细胞数量明显增多 ,酶活性明显增强 ,在酶活性、形态结构和功能上存在异质性变化。交错突细胞数量则明显减少。

Objective To explo re the influence of diabetes on the macrophages and interdigitating cells in rat thymus and the roles of them in the thymus involution.?Methods Using the rat model of alloxan-diabetes and the enzyme-histochenmistry, immuno histochemistry and electron microscopy, qualitative and semi-quantitative analys es and morphologic observation were made on the macrophages and interdigitating cells in thymus.?Results In diabetic rat thymus, the m acrophages were strongly positive for ACP, NSE, Ly and AACT,the number of them increased evidently, groups of the macrophages gathered in the cortex and cortic omedullary zone, especially in the cortex. The interdigitating cells, strongly p ositive for S-100 protein, were evidently reduced in number and mainly distribut ed in the medulla and corticomedullary zone. Electron microscopy revealed apopto tic cells and bodies engulfed by the macrophages in the cortex, but cystic vacuo les and granules engulfed by the macrophages in the corticomedullary zone.?Conclusion In diabetic rat thymus, the macrophages are eviden tly increased in number and enzyme activities, and show heterogenous changes in enzyme activity, morphology and function. The interdigitating cells are evidentl y decreased in number. These cells both may play important roles in the thymus i nvolution.